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Image Search Results
Journal: Clinical cancer research : an official journal of the American Association for Cancer Research
Article Title: Microtubule Engagement with Taxane Is Altered in Taxane-Resistant Gastric Cancer
doi: 10.1158/1078-0432.CCR-19-3018
Figure Lengend Snippet: Differential gene expression analysis of sensitive and resistant cells reveals coordinated modulation of kinesin family is differentially modulated in docetaxel (DTX)-sensitive and docetaxel-resistant DIF-GC cells, which display characteristic microtubule dynamicity. A, Venn diagram representing the DEGs after docetaxel treatment in docetaxel-sensitive (AZ521 and TMK1) and docetaxel-resistant (SCH and MKN7) gastric cancer cell lines. B, Bar graph representing the microtubule-related pathways identified by Reactome pathway analysis of the 84 common DEGs, ranked by significance. C, mRNA expression of the five kinesins was assessed in biopsies obtained from 12 patients with gastric cancer at baseline, with nine matching on-treatment (cabazitaxel) samples. Fold change of on-treatment versus baseline mRNA expression levels was calculated and correlated to response to treatment. D, Computational analysis of microtubule dynamic parameters by direct detection and tracking of GFP-EB1 comets using the MATLAB-based plusTipTracker algorithm. Graphic display of the microtubule growth speed (μm × per minute) in TMK1 and HS746T cell lines at baseline and after 1 hour treatment with increasing concentrations of docetaxel. Mann–Whitney test was used for statistical analysis (*, P < 0.05; **, P < 0.01; n.s., not significant; Ctr, control).
Article Snippet: Image analysis was performed using the
Techniques: Expressing, MANN-WHITNEY